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STEMCELL Technologies Inc
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Image Search Results
Journal: Blood
Article Title: A topological view of human CD34 + cell state trajectories from integrated single-cell output and proteomic data
doi: 10.1182/blood-2018-10-878025
Figure Lengend Snippet: Functionally defined progenitors with NM, L, and/or E potential display partially overlapping molecular profiles. (A-B) Distribution of each progenitor type in t-SNE space. Index sort information was used to map each progenitor to its nearest 10 neighbors in the mass cytometry data based on the scaled intensity of expression of CD45RA, CD71, CD45, CD123, CD34, CD33, CD49f, CD10, CD135, CD38, CD90, HLA-DR, and CD133. The nearest neighbors for all members of a given progenitor type were pooled and used to generate a probability density, indicated by the intensity of the color shown. The lowest level contains 95% of the total probability density, with each higher 10% density levels indicated thereafter. The black contour shows the 75th quantile of the overall density. (A) Mappings for all progenitor types assessed visually in methylcellulose assays as shown in Figure 1B. (B) Mappings for a representative selection of lineage competencies assessed in the STC assays as shown in Figure 1C. (C) A hierarchical clustering of the progenitor types analyzed based on a pairwise assessment of differences in the density distributions between all mappings of functionally and phenotypically defined cell types. Closely related groups are highlighted and given a descriptive name. (D) Multidimensional scaling indicating the relative distances (based on the distribution differences) between all phenotypically and functionally defined progenitor subsets.
Article Snippet: In vitro assays Colony formation in methylcellulose was assessed by single, randomly selected, index-sorted cells deposited directly and individually into the 60 inner wells of a flat bottom Nunc 96-well polystyrene plate (Thermo Fisher Scientific, Waltham, MA) preloaded with 50 µL of
Techniques: Mass Cytometry, Expressing, Selection